Ana Luisa Peluffo made her screen debut in 1953. She was a pioneer to appear in nude scenes in Mexican cinema in mid-decade. This helped her to establish her name and allowed her to turn to dramas and comedies, though she returned to her roots in the 1970s. Peluffo has one of the longest filmographies of any featured actress in Mexican cinema. She has won two Diosas de Plata: one for Best Actress for her role in La venida del rey Olmos (1975), and another for her long career (in 1996).

Ana Luisa Peluffo has appeared nude in La Guerra de los sexos, La Diana cazadora, Corrupción, Perro Callejero 2, Cada quien su madre, El Reventón, Deseos, Perro callejero, Una Rata en la oscuridad, and La Pandilla Motorizada.